Homogeneous assay system
US6214979A · kind A · utility
79Cited by
4References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 19, 1997 |
| Grant date | Apr 10, 2001 |
| Priority date | — |
| Expiry date | Sep 19, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.