Patent · US Expired

Nucleic acid encoding CD28

US6218525A · kind A · utility

7Cited by
1References
16Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 1, 1992
Grant dateApr 17, 2001
Priority date
Expiry dateDec 1, 2012

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2840/44
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

A simple and highly efficient method for cloning cDNAs from mammalian expression libraries based on transient expression in mammalian host cells has been discovered. Novel expression vectors allowing highly efficient construction of mammalian cDNA libraries are disclosed. The cloning method of the invention which has been used to clone genes for cell surface antigens of human lymphocytes, has general application in gene cloning. Cell surface antigens cloned according to the present invention have been purified, and the nucleotide and amino acid sequences determined. These antigens have diagnostic and therapeutic utility in immune-mediated infections in mammals, including humans.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.