Method of recovering a nucleic acid encoding a proteinaceous binding domain which binds a target material

US7118879B2 · kind B2 · utility

Assignee

• Dyax Corp. · US

Inventors

• Robert Charles Ladner · Urbana, US
• Sonia Guterman · Belmont, US
• Bruce Lindsay Roberts · Southborough, US
• William Markland · Milford, US
• Arthur C. Ley · Newton, US
• Rachel Kent · Boxborough, US

Key dates

• Filing date: Apr 22, 2002
• Grant date: Oct 10, 2006
• Expiry date: Mar 16, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2795/14143
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained. In one embodiment, the first family of potential binding domains is related to bovine pancreatic trypsin inhibitor, the genetic package is M13 phage, and the protein includes the outer surface transport signal of the M13 gene III protein.