Process for discrimination of nucleic acid sequence variants
US7141377B2 · kind B2 · utility
30Cited by
17References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 27, 2005 |
| Grant date | Nov 28, 2006 |
| Priority date | — |
| Expiry date | Jan 27, 2025 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.