Homogeneous assay system
US7445900B2 · kind B2 · utility
15Cited by
18References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 11, 2005 |
| Grant date | Nov 4, 2008 |
| Priority date | — |
| Expiry date | Aug 11, 2025 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.