2′-terminator related pyrophosphorolysis activated polymerization
US7745125B2 · kind B2 · utility
14Cited by
24References
32Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 18, 2006 |
| Grant date | Jun 29, 2010 |
| Priority date | — |
| Expiry date | Jan 30, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2′-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2′-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.