2′-Fluoro substituted oligomeric compounds and compositions for use in gene modulations

US7812149B2 · kind B2 · utility

Assignee

• Isis Pharmaceuticals, Inc. · US

Inventors

• Thazha P. Prakash · Carlsbad, US
• Brenda F. Baker · Carlsbad, US
• Anne B. Eldrup · Newtown, US
• Muthiah Manoharan · Carlsbad, US
• Balkrishen Bhat · Carlsbad, US
• Richard H. Griffey · Vista, US
• Eric E. Swayze · Carlsbad, US
• Stanley T. Crooke · Carlsbad, US

Key dates

• Filing date: Nov 4, 2003
• Grant date: Oct 12, 2010
• Expiry date: Nov 4, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2320/51
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions comprising first and second oligomers are provided wherein at least a portion of the first oligomer is capable of hybridizing with at least a portion of the second oligomer, at least a portion of the first oligomer is complementary to and capable of hybridizing to a selected target nucleic acid, and at least one of the first or second oligomers includes a modified sugar and/or backbone modification. In some embodiments the modification is a 2′-F substituent group on a sugar moiety. Oligomer/protein compositions are also provided comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein at least one nucleoside of the oligomer has a modified sugar and/or backbone modification.