Normalization of data

US8089623B2 · kind B2 · utility

Assignee

• Applied Biosystems, LLC · US

Inventors

• H. Pin Kao · Fremont, US
• Ian A. Harding · San Mateo, US
• Achim Karger · Foster City, US
• Mark F. Oldham · Los Gatos, US
• Omead Ostadan · San Leandro, US
• Greg Young · Menlo Park, US

Key dates

• Filing date: Aug 18, 2009
• Grant date: Jan 3, 2012
• Expiry date: Aug 18, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.