System and method for localization of large numbers of fluorescent markers in biological samples
US8319181B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 30, 2011 |
| Grant date | Nov 27, 2012 |
| Priority date | — |
| Expiry date | Jan 30, 2031 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J2237/2857
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and system for the imaging and localization of fluorescent markers such as fluorescent proteins or quantum dots within biological samples is disclosed. The use of recombinant genetics technology to insert “reporter” genes into many species is well established. In particular, green fluorescent proteins (GFPs) and their genetically-modified variants ranging from blue to yellow, are easily spliced into many genomes at the sites of genes of interest (GoIs), where the GFPs are expressed with no apparent effect on the functioning of the proteins of interest (PoIs) coded for by the GoIs. One goal of biologists is more precise localization of PoIs within cells. The invention is a method and system for enabling more rapid and precise PoI localization using charged particle beam-induced damage to GFPs. Multiple embodiments of systems for implementing the method are presented, along with an image processing method relatively immune to high statistical noise levels.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.