Method for direct amplification from crude nucleic acid samples
US8460934B2 · kind B2 · utility
12Cited by
7References
5Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 3, 2012 |
| Grant date | Jun 11, 2013 |
| Priority date | — |
| Expiry date | Apr 3, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.