Method and device for detecting the presence of a single target nucleic acid in a sample

US8563275B2 · kind B2 · utility

Assignees

• Applied Biosystems, LLC · US
• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• James F. Brown · Clearwater Beach, US
• Jonathan E. Silver · Bethesda, US

Key dates

• Filing date: Aug 11, 2012
• Grant date: Oct 22, 2013
• Expiry date: Aug 11, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/2575
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A method comprising subjecting one or more sample portion(s) to a single amplification step, thereby amplifying a single molecule in the sample portion to a detectable level, and, in some embodiments, then determining whether the sample portion contains at least one molecule of the target nucleic acid. In some embodiments, the sample portion is in a porous sample structure, or in a sample chamber which comprises means for minimizing diffusion of the sample portion, or in a sample chamber which is inside a microcapillary device, or in a sample retaining means.