Nucleotide-specific recognition sequences for designer TAL effectors
US8614092B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 31, 2012 |
| Grant date | Dec 24, 2013 |
| Priority date | — |
| Expiry date | Dec 31, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in an animal cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that includes a deoxyribonucleic acid (DNA) binding polypeptide having a N-terminal capping region, a DNA binding domain comprising at least five or more Transcription activator-like effector (TALE) monomers and at least one or more half-monomers specifically ordered to target the genomic locus of interest, and a C-terminal capping region, wherein the polypeptide includes at least one or more effector domains, and wherein the polypeptide is encoded by and translated from a codon optimized nucleic acid molecule so that the polypeptide preferentially binds to the DNA of the genomic locus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.