Method for detecting the presence of a target nucleic acid sequence in a sample
US8859204B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 13, 2007 |
| Grant date | Oct 14, 2014 |
| Priority date | — |
| Expiry date | Sep 12, 2028 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/2575
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method comprises loading a sample portion into a sample chamber which comprises means for minimizing diffusion of the sample portion, subjecting the sample portion to an amplification step, and determining whether the sample portion contains at least one molecule of a target nucleic acid. If the sample portion contains a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. Also, a microfluidic device comprising a sample portion and a sample chamber comprising means for minimizing diffusion of the sample portion. Also, a microfluidic device comprising a sample chamber and an amplification targeting reagent positioned in the first sample chamber.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.