Nucleic acid analyzing device and nucleic acid analyzer
US8865403B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 13, 2009 |
| Grant date | Oct 21, 2014 |
| Priority date | — |
| Expiry date | Aug 7, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/648
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An object of the present invention relates to distinguishing, from a fluorophore of an unreacted substrate, a single fluorophore attached to a nucleotide that is incorporated into a probe by a nucleic acid synthesis. The present invention relates to a nucleic acid analyzing device that analyzes a nucleic acid in sample by fluorescence, wherein a localized surface plasmon is generated by illumination, and a probe for analyzing the nucleic acid in the sample is on the site where the surface plasmon is generated. According to the present invention, since it is possible to efficiently produce fluorescence intensifying effects due to the surface plasmon and to immobilize the probe to a region within the reach of the fluorescence intensifying effects, it becomes possible to measure a nucleic acid synthesis without removing unreacted nucleotide to which fluorophores are attached.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.