CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
US8932814B2 · kind B2 · utility
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30Claims
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Key dates
| Filing date | Apr 22, 2014 |
| Grant date | Jan 13, 2015 |
| Priority date | — |
| Expiry date | Apr 22, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/531
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.