Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains

US8993233B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US
• President and Fellows of Harvard College · US

Inventors

• Feng Zhang · Cambridge, US
• Le Cong · Cambridge, US
• Randall Jeffrey Platt · Cambridge, US
• Neville Espi Sanjana · New York, US
• Fei Ran · Boston, US

Key dates

• Filing date: Dec 12, 2013
• Grant date: Mar 31, 2015
• Expiry date: Dec 12, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/15043
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors with additional functional domains. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.