DNA polymerases and related methods

US9102924B2 · kind B2 · utility

Assignee

• Roche Molecular Systems, Inc. · US

Inventors

• Keith Bauer · Los Altos, US
• Ellen H. Fiss · Albany, US
• David H. Gelfand · Oakland, US
• Edward S. Smith · San Francisco, US
• Shawn Suko · El Sobrante, US
• Olga Budker · Corona, US
• Nancy Schoenbrunner · Pleasanton, US
• Susanne Stoffel · Corona, US
• Thomas W. Myers · Dublin, US

Key dates

• Filing date: Apr 16, 2009
• Grant date: Aug 11, 2015
• Expiry date: Feb 4, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/34
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects by an intercalating dye. Therefore, the mutant polymerases are useful in a variety of disclosed methods in combination with an intercalating dye. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.